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So far you have created a component .dll that holds some validation logic, but you could also create a new .dll that would hold the ReportToFile class that you have been using in the exercises. In fact, you could use all three separate component files for an application. The Validations.dll would be used from a new component file called ReportMaker.dll that held your ReportToFile class; and the BugReporter application would hold your UI code (see Figure 6-7).

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Critical Thinking Question: 1. One way to study mutations that are generally lethal is by isolating temperature-sensitive mutations. These mutations involve amino acids that disrupt the functioning of the enzymes at some critical temperature but are phenotypically normal at other temperatures. Thus, the mutant organisms can be kept alive by growing them at one temperature (the permissive temperature) but their mutant effect can be studied at the temperature in which the protein function is disabled (the restrictive temperature). (For additional discussion of these mutations, see chapter 12.)

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15. A primosome is a helicase plus a primase; it opens the DNA and creates RNA primers on lagging strands and is part of the replisome. A replisome includes a primosome plus two copies of DNA polymerase III; it coordinates replication on both the leading and lagging strands at the Y-junction. 17. See gure 9.28. 19. At one time molecular swivels, presumably protein in nature, located periodically along the DNA, were suggested. 21. RNA RNA DNA

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Figure 6-7. The component files Not all validations have to go into a separate file; if you were making actual testware, you would perform additional testing to determine what validations should be added to one or both component files. If you found validations that would be useful in other testware applications, you would add them to the Validations.dll. If the validations were specific to the ReportMaker.dll, you would only need to add them to that component. If the validations were specific to this particular user interface, you would include them in the BugReporter.exe. For example, say that you want to make sure that the description of the bug is never allowed to be blank. You know that you have a method in the Validations.dll that tests for empty strings. This method returns true when the string is empty and false when it has something in it. So, you would reference the Validations.dll from your ReportMaker.dll and call the TestForEmptyStrings() method as you did in Exercise 6-5. You would use an If statement to determine the outcome of the method and throw an exception when the string was empty (exceptions were covered in 4). Your code would look something like Listing 6-9.

1. See gure 10.3. Complementarity is achieved between messenger RNA and ribosomal RNA and between messenger RNA and transfer RNA. 3. Transcription has higher error rates. Errors of DNA polymerase tend to become permanent, whereas errors of RNA polymerase do not. 5. A consensus sequence is made up of the nucleotides that appear in a signi cant proportion of cases when similar sequences are aligned. A conserved sequence consists of nucleotides found in all cases when similar sequences are aligned. For example, the Pribnow box ( g. 10.6) is the consensus sequence TATAAT. 7. See gure 10.8 for a promoter and gure 10.10 for a terminator. The transcript starting from the promoter would be 5 -CUUAUACGGU....The transcript from the terminator is shown in gure 10.10.

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